1 Extraction of medicinal materials
(1) After passing the tests according to the enterprise standards or national standards, the raw material and supplementary material are prepared for future use;
(2) The extracted drug solutions obtained from (2) are combined, concentrated to a solution of 1.2g raw drug of about 1 g of drug liquid, added with 85% ethanol, allowing the alcohol level at 65 %, placed for 24 h or more, the supernatant was taken out. The ethanol was recovered and concentrated to about 60 ~ 72g (send to testing, the ethanol content should be 2~2.5%).
2 Pretreatment of drug liquid:
The first centrifugation: the TCM concentrated solution collected are put in the refrigeratory (0-5 ℃) to cool down and placed still for more than 24h. Then the first ultracentrifugation (16,000 r / min) was carried out to remove part of the solid substances, those centrifugal liquid was collected to measure at M1 (kg).
二次离心：将一次离心后收集的药液M1，继续放入冷库（0-5℃）冷却并静置24小时以上，进行第二次超速离心（16000转/分钟），弃去固形物部份，收集离心液体，计量为M2千克， 密封后，放置于恒温恒湿库房，0-5℃保存备用。 qnryb.com
The second centrifugation: the TCM solution M1 collected for the first centrifugation was put in the refrigeratory (0-5 ℃) to cool down and placed still for more than 24h. Then the second ultracentrifugation (16,000 r / min) was carried out to remove part of the solid substances, those centrifugal liquid was collected to measure at M2 (kg). After being sealed, the liquid obtained was put in the warehouse of constant temperature and humidity and kept at 0-5℃ for future use.